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Article

Insight into the structural stability of wild type and mutants of the tobacco etch virus protease with molecular dynamics simulations.

Journal of molecular modeling - 1 Nov 2013

Wang Yu, Zhu Guo-Fei, Ren Si-Yan, Han Yong-Guang, Luo Yue, Du Lin-Fang

Abstract excerpt

The efficiency and high specificity of tobacco etch virus protease (TEVp) has made it widely used for cleavage of recombinant fusion proteins. However, TEVp suffers from a few intrinsic defects such as self-cleavage, poorly expressed in E. coli and less soluble. So some mutants were designed to improve it, such as S219V, T17S/N68D/I77V and L56V/S135G etc. MD simulations for the WT TEVp and its mutants were...

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Identifiers

PMID: 24043540
DOI: 10.1007/s00894-013-1930-9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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