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Article

Structural insights into the hydrolysis and polymorphism of methotrexate polyglutamate by zebrafish γ-glutamyl hydrolase.

Journal of medicinal chemistry - 10 Oct 2013

Chuankhayan Phimonphan, Kao Tseng-Ting, Lin Chien-Chih, Guan Hong-Hsiang, Nakagawa Atsushi, Fu Tzu-Fun, Chen Chun-Jung

Abstract excerpt

γ-Glutamyl hydrolases (γGH) catalyze the hydrolysis of γ-linked glutamate residues from the polyglutamyl of folates and antifolates, such as methotrexate (MTX), a widely used anticancer drug. We describe the first crystal structures of the endopeptidase-type γGH (zγGH) from zebrafish and the mutant complexes with MTX(Glu)5 and hydrolyzed MTX(Glu)1, revealing the complete set of key residues involved in hydrolysis...

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PMID: 24028568
DOI: 10.1021/jm401013e

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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