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Article

Kinetic characterization of two active mutants of placental ribonuclease inhibitor that lack internal repeats.

Biochemistry - 17 Jul 1990

Lee F S, Vallee B L

Abstract excerpt

Human placental ribonuclease inhibitor (PRI), a 50-kDa tight-binding inhibitor of angiogenin and pancreatic ribonuclease, consists predominantly of 7 internal repeats, each 57 residues long. Repeats 3 plus 4 (residues 144-257) or repeat 6 (residues 315-371) can be deleted to give mutant proteins, PRI delta 3-4 and PRI delta 6, respectively, that retain inhibitory activity [Lee, F. S., & Vallee, B. L. (1990) Proc....

Topics

Base Sequence
Binding Sites
Humans
Kinetics
Molecular Sequence Data
Mutation
Placental Hormones
Protein Binding
Protein Conformation
Protein Engineering
Proteins
Recombinant Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Repetitive Sequences, Nucleic Acid

Ribonuclease, Pancreatic

Source

Identifiers

PMID: 2397204
DOI: 10.1021/bi00480a012

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2397204-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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