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Article

Characterization of recombinant human protein C inhibitor expressed in Escherichia coli.

Biochimica et biophysica acta - 15 Apr 2005

Réhault Sophie M, Zechmeister-Machhart Margareta, Fortenberry Yolanda M, Malleier Julia, Binz Nikki M, Cooper Scott T, Geiger Margarethe, Church Frank C

Abstract excerpt

The serine protease inhibitor (serpin) protein C inhibitor (PCI; also named plasminogen activator inhibitor-3) regulates serine proteases in hemostasis, fibrinolysis, and reproduction. The biochemical activity of PCI is not fully defined partly due to the lack of a convenient expression system for active rPCI. Using pET-15b plasmid, Ni(2+)-chelate and heparin-Sepharose affinity chromatography steps, we describe...

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Identifiers

PMID: 15752693
DOI: 10.1016/j.bbapap.2004.12.003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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