Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Sensitive, simultaneous quantitation of two unlabeled DNA targets using a magnetic nanoparticle-enzyme sandwich assay.

Analytical chemistry - 1 Oct 2013

Zhang Yue, Pilapong Chalermchai, Guo Yuan, Ling Zhenlian, Cespedes Oscar, Quirke Philip, Zhou Dejian

Abstract excerpt

We report herein the development of a simple, sensitive colorimetric magnetic nanoparticle (MNP)-enzyme-based DNA sandwich assay that is suitable for simultaneous, label-free quantitation of two DNA targets down to 50 fM level. It can also effectively discriminate single-nucleotide polymorphisms (SNPs) in genes associated with human cancers (KRAS codon 12/13 SNPs). This assay uses a pair of specific DNA probes,...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biosensing Techniques
Share to Colorimetry

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA, Neoplasm

Share to DNA Probes

Share to Enzymes

Share to Fluorescent Dyes

Share to Molecular Structure

Share to Polymorphism, Single Nucleotide

Source

Open in PubMed Open full text

Identifiers

PMID: 23971744
DOI: 10.1021/ac402081u
PMCID: PMC3789254

Genes

KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Sensitive, simultaneous quantitation of two unlabeled DNA targets using a magnetic nanoparticle-enzyme sandwich assay.PMID 23971744Read ↗

Ultrasensitive single-nucleotide polymorphism detection using target-recycled ligation, strand displacement and enzymatic amplification.Semantic proximity Read ↗

Highly sensitive and selective colorimetric genotyping of single-nucleotide polymorphisms based on enzyme-amplified ligation on magnetic beads.Semantic proximity Read ↗

A novel automated assay with dual-color hybridization for single-nucleotide polymorphisms genotyping on gold magnetic nanoparticle array.Semantic proximity Read ↗

A dynamic sandwich assay on magnetic beads for selective detection of single-nucleotide mutations at room temperature.Semantic proximity Read ↗

High-throughput SNP detection based on PCR amplification on magnetic nanoparticles using dual-color hybridization.Semantic proximity Read ↗

Rapid KRAS Mutation Detection via Hybridization-Induced Aggregation of Microbeads.Semantic proximity Read ↗

High-throughput SNP genotyping based on solid-phase PCR on magnetic nanoparticles with dual-color hybridization.Semantic proximity Read ↗

Magnetic concentration of allele-specific products from recombinase polymerase amplification.Semantic proximity Read ↗

Chemiluminescence resonance energy transfer imaging on magnetic particles for single-nucleotide polymorphism detection based on ligation chain reaction.Semantic proximity Read ↗

Simple, rapid, homogeneous oligonucleotides colorimetric detection based on non-aggregated gold nanoparticles.Semantic proximity Read ↗

Ultraspecific Multiplexed Detection of Low-Abundance Single-Nucleotide Variants by Combining a Masking Tactic with Fluorescent Nanoparticle Counting.Semantic proximity Read ↗

In-Situ Mutation Detection by Magnetic Beads-Probe Based on Single Base Extension and Its Application in Genotyping of Hepatitis B Virus Pre-C Region 1896nt Locus Single Nucleotide Polymorphisms.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.