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Article

A 22-nt artificial microRNA mediates widespread RNA silencing in Arabidopsis.

The Plant journal : for cell and molecular biology - 1 Nov 2013

McHale Marcus, Eamens Andrew L, Finnegan E Jean, Waterhouse Peter M

Abstract excerpt

It is known that 22-nucleotide (nt) microRNAs (miRNAs) derived from asymmetric duplexes trigger phased small-interfering RNA (phasiRNA) production from complementary targets. Here we investigate the efficacy of 22-nt artificial miRNA (amiRNA)-mediated RNA silencing relative to conventional hairpin RNA (hpRNA) and 21-nt amiRNA-mediated RNA silencing. CHALCONE SYNTHASE (CHS) was selected as a target in Arabidopsis...

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Identifiers

PMID: 23937661
DOI: 10.1111/tpj.12306
PMCID: PMC4241025

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