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Article

Undesired small RNAs originate from an artificial microRNA precursor in transgenic petunia (Petunia hybrida).

PloS one - 1 Jan 2014

Guo Yulong, Han Yao, Ma Jing, Wang Huiping, Sang Xianchun, Li Mingyang

Abstract excerpt

Although artificial microRNA (amiRNA) technology has been used frequently in gene silencing in plants, little research has been devoted to investigating the accuracy of amiRNA precursor processing. In this work, amiRNAchs1 (amiRchs1), based on the Arabidopsis miR319a precursor, was expressed in order to suppress the expression of CHS genes in petunia. The transgenic plants showed the CHS gene-silencing phenotype....

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 24897430
DOI: 10.1371/journal.pone.0098783
PMCID: PMC4045805

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