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Article

hpRNA-mediated targeting of the Arabidopsis FAD2 gene gives highly efficient and stable silencing.

Plant physiology - 1 Aug 2002

Stoutjesdijk Peter A, Singh Surinder P, Liu Qing, Hurlstone Clive J, Waterhouse Peter A, Green Allan G

Abstract excerpt

The endogenous Delta 12-desaturase gene (FAD2) in Arabidopsis was targeted for silencing using seed-specific cosuppression (CS), hairpin (HP) RNA (hpRNA), and intron-spliced HP (iHP) constructs. The iHP construct, incorporating the 120-bp 3'-untranslated region of the FAD2 gene, gave the highest degree of silencing. In some iHP lines Delta 12-desaturase activity was reduced to levels as low as those in the null...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 12177485
DOI: 10.1104/pp.006353
PMCID: PMC166760

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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