Article
Multi-gene silencing in Arabidopsis: a collection of artificial microRNAs targeting groups of paralogs encoding transcription factors.
The Plant journal : for cell and molecular biology - 1 Oct 2014
Jover-Gil Sara, Paz-Ares Javier, Micol José Luis, Ponce María Rosa
Abstract excerpt
Functional redundancy often hampers the analysis of gene families. To overcome this difficulty, we constructed Arabidopsis thaliana lines that expressed artificial microRNAs designed to simultaneously target two to six paralogous genes encoding members of transcription factor families. Of the 576 genes that we chose as targets, only 122 had already been functionally studied at some level. As a simple indicator of...
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