Simple and rapid human papillomavirus genotyping method by restriction fragment length polymorphism analysis with two restriction enzymes.

Abstract excerpt

Cervical cancer, the third most common cancer that affects women worldwide, is caused by the human papillomavirus (HPV) and is treatable when detected at an early stage. To date, more than 100 different HPV types have been described, and the development of simple, low-cost, and accurate methods to distinguish HPV genotypes is highly warranted. In this study, an HPV genotyping assay based on polymerase chain...

Topics

• DNA Restriction Enzymes
• DNA, Viral
• Female
• Genotype
• Humans
• Papillomaviridae
• Papillomavirus Infections
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length
• Uterine Cervical Neoplasms