Article
Full HPV typing by a single restriction enzyme.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 1 Sept 2006
Santiago Enrique, Camacho Lucía, Junquera Maria Luisa, Vázquez Fernando
Abstract excerpt
BACKGROUND: Restriction fragment length polymorphism (RFLP) methods for genotyping genital human papillomavirus (HPV) are considered labor consuming and constrained by the reduced set of restriction enzymes capable of detecting specific mutations. However, we think that these methods have not taken full advantage of the high diversity of the known restriction enzymes. OBJECTIVE: We have set out to find the best...
Topics
- Benzothiazoles
- Capsid Proteins
- DNA Restriction Enzymes
- DNA, Viral
- Diamines
- Electrophoresis, Polyacrylamide Gel
- Female
- Fluorescent Dyes
- Genotype
- Humans
- Oncogene Proteins, Viral
- Organic Chemicals
- Papillomaviridae
- Polymorphism, Restriction Fragment Length
- Quinolines
- Staining and Labeling
- Virology
