Article
A novel and rapid PCR-based method for genotyping human papillomaviruses in clinical samples.
Journal of clinical microbiology - 1 Feb 2000
Nelson J H, Hawkins G A, Edlund K, Evander M, Kjellberg L, Wadell G, Dillner J, Gerasimova T, Coker A L, Pirisi L, Petereit D, Lambert P F
Abstract excerpt
Many human papillomavirus (HPV) genotypes are associated with cervical carcinoma. We demonstrate the utility of an innovative technique for genotyping of HPV in cervical tissue samples. This method provides an accurate means of identification of the specific HPV genotypes present in clinical specimens. By using the MY09-MY11 and the GP5(+)-GP6(+) consensus primer pairs, HPV sequences were amplified by nested PCR...
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