Article
Herpes simplex virus genotyping: multiple optional PCR-based RFLP systems and a non-isotopic single-strand conformation polymorphism method.
Journal of virological methods - 1 Jun 2004
Abba Martín C, Golijow Carlos D
Abstract excerpt
Polymerase chain reaction (PCR)-based methods for herpes simplex virus (HSV) types I/II genotyping are described. These methods are based on the single-stranded conformation properties of DNA molecules obtained by PCR (PCR-SSCP) and restriction fragment analysis of PCR products (PCR-RFLP). With the aim to analyse these two genotyping techniques, genomic DNA from the standard viral strains KOS and G, and also 79...
Topics
- Base Sequence
- Cervix Uteri
- DNA, Viral
- Female
- Genotype
- Herpesvirus 1, Human
- Herpesvirus 2, Human
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Polymorphism, Single-Stranded Conformational
- Sequence Homology, Amino Acid
- Virology
