Article
Detection and identification of human papillomavirus using a PCR-restriction fragment mass polymorphism assay.
Molecular medicine reports - 1 Jan 2000
Lee Jin Kyung, Hong Young Jun, Um Tae Hyun, Lee Eun Hee, Chi Hyun-Sook, Koh Jae Soo, Yim Hyeon Woo, Cha Young Joo
Abstract excerpt
A polymerase chain reaction-restriction fragment mass polymorphism (PCR-RFMP) assay protocol using PGMY09/11 primers for the detection and identification of human papillomavirus (HPV) has recently been developed. The present study evaluated the analytical sensitivity and clinical utility of HPV genotyping employing PCR-RFMP as compared to direct sequencing. Serial dilutions of cloned HPV DNA were analyzed in...
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