Expression, purification and crystallization of penicillin G acylase from Escherichia coli ATCC 11105.

doi:10.1093/protein/3.7.635

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Article

Expression, purification and crystallization of penicillin G acylase from Escherichia coli ATCC 11105.

Protein engineering - 1 Jul 1990

Hunt P D, Tolley S P, Ward R J, Hill C P, Dodson G G

Abstract excerpt

The penicillin acylase gene (pac) from Escherichia coli ATCC 11105 was cloned into pUC 9 and the resulting vector (pUPA-9), when transformed into E. coli strain 5K, allowed the constitutive overproduction of mature penicillin acylase when grown at 28 degrees C. The enzyme was purified from the pe...

Topics

Chromatography, Ion Exchange
Cloning, Molecular
Escherichia coli
Mutation
Penicillin Amidase
Solubility
X-Ray Diffraction

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