Article
Zygosity determination in hairless mice by PCR based on Hr(hr) gene analysis.
Experimental animals - 1 Jan 2013
Suzuki Osamu, Koura Minako, Noguchi Yoko, Uchio-Yamada Kozue, Matsuda Junichiro
Abstract excerpt
We analyzed the Hr gene of a hairless mouse strain of unknown origin (HR strain, http://animal.nibio.go.jp/e_hr.html) to determine whether the strain shares a mutation with other hairless strains, such as HRS/J and Skh:HR-1, both of which have an Hr(hr) allele. Using PCR with multiple pairs of primers designed to amplify multiple overlapping regions covering the entire Hr gene, we found an insertion mutation in...
Topics
- Aging
- Alleles
- Animals
- Female
- Genome
- Genotyping Techniques
- Heterozygote
- Homozygote
- Male
- Mice
- Mice, Hairless
- Mutation
- Polymerase Chain Reaction
- Transcription Factors