Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Affinity maturation of a CDR3-grafted VHH using in silico analysis and surface plasmon resonance.

Journal of biochemistry - 1 Oct 2013

Inoue Hidetoshi, Suganami Akiko, Ishida Isao, Tamura Yutaka, Maeda Yoshitake

Abstract excerpt

A requirement for advancing antibody-based medicine is the development of proteins that can bind with high affinity to a specific epitope related to a critical protein activity site. As a part of generating such proteins, we have succeeded in creating a binding protein without changing epitope by...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acid Sequence
Share to Camelids, New World
Share to Computer Simulation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Models, Molecular

Share to Molecular Sequence Data

Share to Recombinant Proteins

Share to Single-Domain Antibodies

Share to Surface Plasmon Resonance

Source

Identifiers

PMID: 23902829
DOI: 10.1093/jb/mvt058

Genes

CDR2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-23902829-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Affinity maturation of a CDR3-grafted VHH using in silico analysis and surface plasmon resonance.PMID 23902829Read ↗

Molecular basis for thermal stability and affinity in a VHH: Contribution of the framework region and its influence in the conformation of the CDR3Semantic proximity Read ↗

Characterization of a high‐affinity human antibody with a disulfide bridge in the third complementarity‐determining region of the heavy chainSemantic proximity Read ↗

Humanization of antibodies using heavy chain complementarity-determining region 3 grafting coupled with in vitro somatic hypermutation.Semantic proximity Read ↗

A single-domain antibody library based on a stability-engineered human VH3 scaffoldSemantic proximity Read ↗

Improving antibody affinity through in vitro mutagenesis in complementarity determining regionsSemantic proximity Read ↗

Affinity maturation by semi-rational approaches.Semantic proximity Read ↗

AI-Guided Precision in Antibody Humanization: Structural Modeling to Minimize Immunogenicity and Preserve EfficacySemantic proximity Read ↗

Protein engineering approaches for antibody fragments: directed evolution and rational design approachesSemantic proximity Read ↗

Antibody humanization methods – a review and updateSemantic proximity Read ↗

Affinity Maturation for Antibody Engineering: The Critical Role of Residues on CDR Loops of Antibodies in Antigen BindingSemantic proximity Read ↗

Heavy chain-only antibodies with a stabilized human VH in transgenic chickens for therapeutic antibody discoverySemantic proximity Read ↗

A structure-based engineering approach to abrogate pre-existing antibody binding to biotherapeuticsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.