Article
[Study on TaqMan-MGB real-time fluorescence quantitative PCR to detect gene mutation of kdr from Anopheles sinensis].
Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control - 1 Apr 2013
Bai Liang, Zhu Guo-Ding, Tang Jian-Xia, Zhang Chao, Liu Yao-Bao, Li Ju-Lin, Cao Jun, Gao Qi
Abstract excerpt
OBJECTIVE: To establish a Real-time Fluorescence Quantitative PCR to detect the kdr gene mutation in Anopheles sinensis. METHODS: One pair of primers and three TaqMan-MGB probes were designed based on kdr gene and its L1014 locus mutations of A. sinensis. After optimization, the Real-time Fluorescence Quantitative PCR was verified by using 6 types of A. sinensis samples with different kdr gene types....
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