Article
High-throughput approach to detection of knockdown resistance (kdr) mutation in mosquitoes, Culex quinquefasciatus, based on real-time PCR using single-labelled hybridisation probe/melting curve analysis.
Pest management science - 1 Feb 2011
Sarkar Manas, Baruah Indra, Srivastava Ravi B, Borkotoki Aparajita, Bhattacharyya Indra Kumar
Abstract excerpt
BACKGROUND: Knockdown resistance (kdr) mutation (L1014F) is a well-defined mechanism of resistance to pyrethroids and DDT in many insect species. Sensitive detection of the mutations associated with resistance is a prerequisite for resistance management strategies. The authors have developed a new real-time molecular diagnostic assay based on SimpleProbe(®)/melting curve analysis for large-scale kdr genotyping in...
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