Article
Impaired replication elongation in Tetrahymena mutants deficient in histone H3 Lys 27 monomethylation.
Genes & development - 1 Aug 2013
Gao Shan, Xiong Jie, Zhang Chunchao, Berquist Brian R, Yang Rendong, Zhao Meng, Molascon Anthony J, Kwiatkowski Shaina Y, Yuan Dongxia, Qin Zhaohui, Wen Jianfan, Kapler Geoffrey M, Andrews Philip C, Miao Wei, Liu Yifan
Abstract excerpt
Replication of nuclear DNA occurs in the context of chromatin and is influenced by histone modifications. In the ciliate Tetrahymena thermophila, we identified TXR1, encoding a histone methyltransferase. TXR1 deletion resulted in severe DNA replication stress, manifested by the accumulation of ssDNA, production of aberrant replication intermediates, and activation of robust DNA damage responses. Paired-end...
Topics
- DNA Replication
- DNA, Single-Stranded
- Histones
- Methylation
- Mutation
- Proliferating Cell Nuclear Antigen
- Repressor Proteins
- Tetrahymena thermophila