Article
Optimizing SNP microarray probe design for high accuracy microbial genotyping.
Journal of microbiological methods - 1 Sept 2013
Gardner Shea N, Thissen James B, McLoughlin Kevin S, Slezak Tom, Jaing Crystal J
Abstract excerpt
Microarrays to characterize single nucleotide polymorphisms (SNPs) provide a cost-effective and rapid method (under 24h) to genotype microbes as an alternative to sequencing. We developed a pipeline for SNP discovery and microarray design that scales to 100's of microbial genomes. Here we tested various SNP probe design strategies against 8 sequenced isolates of Bacillus anthracis to compare sequence and...
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