Article
Colorimetric detection of DNA sequences using an organic solvent to induce the aggregation of label-free gold nanoparticles.
Journal of nanoscience and nanotechnology - 1 Jun 2013
Jia Huning, Cao Xiaomei, Fu Cong, Wang Jianping, Chen Zhiyao, Zou Bingjie, Jin Li, Yan Zhengyu, Zhou Guohua
Abstract excerpt
A novel assay based on a solvent for inducing the aggregation of AuNPs colloid was proposed to discriminate ssDNA from dsDNA. Eleven organic solvents with different polarity were investigated, and it was found that DMSO was possible to aggregate AuNPs at the amount of only 0.4 microL in a 50-microL detection system. Further research showed that 0.8 microL of DMSO could discriminate the ssDNA from dsDNA....
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