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Article

Development of a PCR/LDR/flow-through hybridization assay using a capillary tube, probe DNA-immobilized magnetic beads and chemiluminescence detection.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry - 1 Jan 2013

Hommatsu Manami, Okahashi Hisamitsu, Ohta Keisuke, Tamai Yusuke, Tsukagoshi Kazuhiko, Hashimoto Masahiko

Abstract excerpt

A polymerase chain reaction (PCR)/ligase detection reaction (LDR)/flow-through hybridization assay using chemiluminescence (CL) detection was developed for analyzing point mutations in gene fragments with high diagnostic value for colorectal cancers. A flow-through hybridization format using a capillary tube, in which probe DNA-immobilized magnetic beads were packed, provided accelerated hybridization kinetics of...

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PMID: 23842410
DOI: 10.2116/analsci.29.689

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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