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Article

A multiplex qPCR gene dosage assay for rapid genotyping and large-scale population screening for deletional α-thalassemia.

The Journal of molecular diagnostics : JMD - 1 Sept 2013

Zhou Wanjun, Wang Ge, Zhao Xuefeng, Xiong Fu, Zhou Shaoxiong, Peng Jianming, Cheng Youming, Xu Shun, Xu Xiangmin

Abstract excerpt

The predominant determinants of α-thalassemia are deletions in the human α-globin gene cluster. A rapid DNA-based assay is needed for mass screening in thalassemia-prevention programs. Herein, we established a novel quadruplex TaqMan qPCR gene dosage assay with two separate combination reactions. The assay directly determined the copy number of human α-globin genes based on relative quantitation of three target...

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Source

Identifiers

PMID: 23810501
DOI: 10.1016/j.jmoldx.2013.05.007

Genes

CREBBP
HBA1
HBA2
HBZ

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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