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Article

Detection of the hemoglobin E mutation using the color complementation assay: application to complex genotyping.

Blood - 1 Aug 1990

Embury S H, Kropp G L, Stanton T S, Warren T C, Cornett P A, Chehab F F

Abstract excerpt

The color complementation assay (CCA) is a method of allele-specific DNA amplification by which competitive priming and extension of fluorescently labeled oligonucleotide primers determine the color of DNA amplification product. This diagnostic method precludes the need for radioisotopes, electrophoresis, and multiple high-stringency reaction conditions. The multiplicity of mutant globin genes present in...

Topics

Adult
DNA
Electrophoresis
Female
Gene Amplification
Genetic Counseling
Genotype
Hemoglobin E
Hemoglobins, Abnormal
Humans
Mutation
Nucleic Acid Amplification Techniques

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Rhodamines

Spectrometry, Fluorescence

Thalassemia

Source

Identifiers

PMID: 2378988

Genes

SEA

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2378988-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Detection of the hemoglobin E mutation using the color complementation assay: application to complex genotyping.PMID 2378988Read ↗

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