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Article

A simple non radioactive method for detecting beta-thalassemia/hbe disease: application to prenatal diagnosis.

The Southeast Asian journal of tropical medicine and public health - 1 Jan 1995

Fucharoen S, Fucharoen G, Ratanasiri T, Jetsrisuparb A, Fukumaki Y

Abstract excerpt

We have developed allele specific polymerase chain reaction (ASPCR) that allows rapid screening of the beta E-globin and common beta-thalassemia genes in Thailand. These non-radioactive methods are based on the amplification by the polymerase chain reaction of the beta E and beta-thalassemia specific DNA fragments using specific primers. With this approach, both heterozygote and homozygote for the disease could...

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PMID: 8629124

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