Article
A simple non radioactive method for detecting beta-thalassemia/hbe disease: application to prenatal diagnosis.
The Southeast Asian journal of tropical medicine and public health - 1 Jan 1995
Fucharoen S, Fucharoen G, Ratanasiri T, Jetsrisuparb A, Fukumaki Y
Abstract excerpt
We have developed allele specific polymerase chain reaction (ASPCR) that allows rapid screening of the beta E-globin and common beta-thalassemia genes in Thailand. These non-radioactive methods are based on the amplification by the polymerase chain reaction of the beta E and beta-thalassemia spec...
Topics
- Alleles
- Base Sequence
- DNA Primers
- Female
- Genetic Carrier Screening
- Globins
- Hemoglobin E
- Hemoglobinuria
- Homozygote
- Humans
- Male
- Molecular Sequence Data
- Mutation
- Pedigree
- Polymerase Chain Reaction
- Pregnancy
- Prenatal Diagnosis
- Risk Assessment