Article
Detection of Hb E mutation (beta(26), GAG-AAG, Glu-Lys) using allelic discrimination analysis.
International journal of laboratory hematology - 1 Feb 2009
Sangkitporn S, Sangkitporn S K, Sangnoi A, Duangruang S
Abstract excerpt
A method for detection of hemoglobin (Hb) E mutation was developed based on allelic discrimination analysis. Two probes labeled with different fluorescent reporter dyes were designed to specifically detect variation of a single nucleic acid polymorphism (SNP) site in the target template sequence. Polymerase chain reaction (PCR) products of normal allele and mutant allele were detected directly by analyzing...
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