Article
Detection and comparison of EGFR mutations in matched tumor tissues, cell blocks, pleural effusions, and sera from patients with NSCLC with malignant pleural effusion, by PNA clamping and direct sequencing.
Lung cancer (Amsterdam, Netherlands) - 1 Aug 2013
Yeo Chang Dong, Kim Jin Woo, Kim Kwan Hyoung, Ha Jick Hwan, Rhee Chin Kook, Kim Seung Joon, Kim Young Kyoon, Park Chan Kwon, Lee Sang Haak, Park Mi Sun, Yim Hyeon Woo
Abstract excerpt
Peptide nucleic acid (PNA)-mediated real-time PCR clamping has higher sensitivity than conventional direct sequencing for detecting mutations. Pleural effusion and serum may provide good samples in which to detect epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer (NSCLC) patients. We studied 37 NSCLC patients with malignant pleural effusion. EGFR mutations were assessed by PNA...
Topics
- Adult
- Aged
- Carcinoma, Non-Small-Cell Lung
- ErbB Receptors
- Female
- Humans
- Lung Neoplasms
- Male
- Middle Aged
- Mutation