Article
Neural induction with a dopaminergic phenotype from human pluripotent stem cells through a feeder-free floating aggregation culture.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013
Morizane Asuka, Doi Daisuke, Takahashi Jun
Abstract excerpt
Pluripotent stem cells are promising potential sources for cell replacement therapy and are useful research tools for exploring disease mechanisms. Neural cells are one of the cell types that have been most efficiently differentiated through several established protocols. This chapter describes the feeder-free floating aggregation culture system for the induction of dopaminergic neurons. This method is simple and...
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