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Article

An Efficient Low Cost Method for Gene Transfer to T Lymphocytes

26 Mar 2013

Abstract excerpt

UNLABELLED: Gene transfer to T lymphocytes has historically relied on retro and lentivirus, but recently transposon-based gene transfer is rising as a simpler and straight forward approach to achieve stable transgene expression. Transfer of expression cassettes to T lymphocytes remains challenging, being based mainly on commercial kits. AIMS: We herein report a convenient and affordable method based on in house...

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PMID: 23555950
DOI: 10.1371/journal.pone.0060298

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