Article

Novel and Efficient Plasmid Transfection Protocols for Functional Analysis of Genetic Elements in Human Cardiac Fibroblasts

2024-05-21

Abstract excerpt

Cardiac fibroblasts, have lower gene transfer efficiency compared to dermal fibroblasts, posing challenges for plasmid-based gene transfer methods. A higher transfer efficiency could enable improved insight into heart pathology and development of novel therapeutic targets. In this study we compared eleven commercially available transfection reagents and eight plasmid purification methods. Finally, we systematicall...

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Literature Corpus work: 87fa2bf4-53f5-5960-952c-4ecd1d7b0db4
DOI: 10.1101/2024.05.19.594879

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