Article
A convenient qualitative and quantitative method to investigate RHD-RHCE hybrid genes.
Transfusion - 1 Nov 2013
Fichou Yann, Le Maréchal Cédric, Bryckaert Laurence, Dupont Isabelle, Jamet Déborah, Chen Jian-Min, Férec Claude
Abstract excerpt
BACKGROUND: Molecular biology techniques, such as single specific-primer polymerase chain reaction (PCR), denaturing-high performance liquid chromatography, direct sequencing, next-generation sequencing, and microarray platforms, contribute to the efficient genotyping of the human blood group RHD gene. However, some alleles remain undetermined in rare cases in DNA samples carrying two copies of the RHD gene,...
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