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Complete RHD next-generation sequencing: establishment of reference RHD alleles

18 Oct 2018

Abstract excerpt

Abstract The Rh blood group system (ISBT004) is the second most important blood group after ABO and is the most polymorphic one, with 55 antigens encoded by 2 genes, RHD and RHCE. This research uses next-generation sequencing (NGS) to sequence the complete RHD gene by amplifying the whole gene using overlapping long-range polymerase chain reaction (LR-PCR) amplicons. The aim was to study different RHD alleles...

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PMID: 30337299
DOI: 10.1182/bloodadvances.2018017871

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