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Article

Rapid detection of TEM-type extended-spectrum β-lactamase (ESBL) mutations using lights-on/lights-off probes with single-stranded DNA amplification.

The Journal of molecular diagnostics : JMD - 1 May 2013

Pierce Kenneth E, Peter Harald, Bachmann Till T, Volpe Carmelo, Mistry Rohit, Rice John E, Wangh Lawrence J

Abstract excerpt

Rapid identification of specific TEM-type β-lactamase genes in bacterial infections is important for determining appropriate clinical treatment. We report here the design and initial testing of a molecular diagnostic assay capable of amplifying a large segment of the blaTEM gene, as well as detecting widely spaced extended-spectrum β-lactamase (ESBL) mutations and inhibitor-resistant TEM (IRT) mutations (eg,...

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Identifiers

PMID: 23518216
DOI: 10.1016/j.jmoldx.2013.02.002

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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