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Efficient Direct Extended-Spectrum β-Lactamase Detection by Multiplex Real-Time PCR: Accurate Assignment of Phenotype by Use of a Limited Set of Genetic Markers

26 May 2011

Abstract excerpt

The number and diversity of genes potentially complicate genetic approaches to the rapid detection of transmissible extended-spectrum β-lactamase genes. We developed a robust multiplexed real-time PCR assay based on targets identified in a prior survey and used this to detect relevant genes in 617 consecutive clinical isolates of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae.

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 21613435
DOI: 10.1128/jcm.02647-10

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