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Article

Development of a Multiplex Real-Time PCR Assay for Mycobacterium bovis BCG and Validation in a Clinical Laboratory.

Microbiology spectrum - 31 Oct 2021

Duffy Shannon C, Venkatesan Manigandan, Chothe Shubhada, Poojary Indira, Verghese Valsan Philip, Kapur Vivek, Behr Marcel A, Michael Joy Sarojini

Abstract excerpt

Mycobacterium bovis bacillus Calmette-Guérin (BCG) is a live attenuated vaccine which can result in local or disseminated infection, most commonly in immunocompromised individuals. Differentiation of BCG from other members of the Mycobacterium tuberculosis complex (MTBC) is required to diagnose BCG disease, which requires specific management. Current methods for BCG diagnosis are based on mycobacterial culture...

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Identifiers

PMID: 34494864
DOI: 10.1128/Spectrum.01098-21
PMCID: PMC8557883

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