Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Improving Escherichia coli FucO for furfural tolerance by saturation mutagenesis of individual amino acid positions.

Applied and environmental microbiology - 1 May 2013

Zheng Huabao, Wang Xuan, Yomano Lorraine P, Geddes Ryan D, Shanmugam Keelnatham T, Ingram Lonnie O

Abstract excerpt

Furfural is an inhibitory side product formed during the depolymerization of hemicellulose with mineral acids. In Escherichia coli, furfural tolerance can be increased by expressing the native fucO gene (encoding lactaldehyde oxidoreductase). This enzyme also catalyzes the NADH-dependent reduction of furfural to the less toxic alcohol. Saturation mutagenesis was combined with growth-based selection to isolate a...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Adaptation, Biological
Share to Alcohol Oxidoreductases

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Fermentation

Share to Gene Expression Regulation, Enzymologic

Share to Leucine

Share to Protein Multimerization

Share to RNA, Bacterial

Share to RNA Folding

Source

Open in PubMed Open full text

Identifiers

PMID: 23475621
DOI: 10.1128/AEM.00149-13
PMCID: PMC3685246

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Improving Escherichia coli FucO for furfural tolerance by saturation mutagenesis of individual amino acid positions.PMID 23475621Read ↗

Engineering an allosteric transcription factor to respond to new ligands.Semantic proximity Read ↗

Beneficial base substitutions in Escherichia coli fucO gene for enhancement of glycolic acid production.Semantic proximity Read ↗

Evolution of an Escherichia coli protein with increased resistance to oxidative stress.Semantic proximity Read ↗

A cyclic AMP receptor protein mutant that constitutively activates an Escherichia coli promoter disrupted by an IS5 insertion.Semantic proximity Read ↗

Identification of an archaeal alpha-L-fucosidase encoded by an interrupted gene. Production of a functional enzyme by mutations mimicking programmed -1 frameshifting.Semantic proximity Read ↗

Characterization of the Vibrio alginolyticusfur gene and localization of essential amino acid sites in fur by site-directed mutagenesis.Semantic proximity Read ↗

Characterization of transmembrane domains 6, 7, and 8 of MalF by mutational analysis.Semantic proximity Read ↗

Overexpression, purification, and generation of a thermostable variant of Zymomonas mobilis fructokinase.Semantic proximity Read ↗

Sugar-binding and crystallographic studies of an arabinose-binding protein mutant (Met108Leu) that exhibits enhanced affinity and altered specificity.Semantic proximity Read ↗

Site-directed mutagenesis of pseudoazurin from Alcaligenes faecalis S-6; Pro80Ala mutant exhibits marked increase in reduction potential.Semantic proximity Read ↗

The amino acid sequence of ribitol dehydrogenase-F, a mutant enzyme with improved xylitol dehydrogenase activity.Semantic proximity Read ↗

Polymerase and hydrolase activities of Bacillus subtilis levansucrase can be separately modulated by site-directed mutagenesis.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.