Article
Functional differences between Streptococcus pyogenes cluster 1 and cluster 2b streptokinases are determined by their β-domains.
FEBS letters - 2 May 2013
Zhang Yueling, Liang Zhong, Glinton Kristofor, Ploplis Victoria A, Castellino Francis J
Abstract excerpt
Cluster 1 streptokinases (SK1) from Streptococcus pyogenes (GAS) show substantially higher human plasminogen (hPg) activation activities and tighter hPg binding affinities than cluster 2b streptokinases (SK2b) in solution. The extent to which the different domains of SK are responsible for these differences is unknown. We exchanged each of the three known SK domains (α, β, and γ) between SK1 and SK2b and assessed...
Topics
- Amino Acid Sequence
- Conserved Sequence
- Enzyme Activation
- Humans
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Plasminogen
- Protein Structure, Secondary
- Protein Structure, Tertiary
- Streptococcus pyogenes
- Streptokinase
- Structure-Activity Relationship