Article
Next-generation sequencing to inventory taxonomic diversity in eukaryotic communities: a test for freshwater diatoms.
Molecular ecology resources - 1 Jul 2013
Kermarrec L, Franc A, Rimet F, Chaumeil P, Humbert J F, Bouchez A
Abstract excerpt
The recent emergence of barcoding approaches coupled to those of next-generation sequencing (NGS) has raised new perspectives for studying environmental communities. In this framework, we tested the possibility to derive accurate inventories of diatom communities from pyrosequencing outputs with an available DNA reference library. We used three molecular markers targeting the nuclear, chloroplast and...
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