Article
Genome-wide ENU mutagenesis in combination with high density SNP analysis and exome sequencing provides rapid identification of novel mouse models of developmental disease.
PloS one - 1 Jan 2013
Caruana Georgina, Farlie Peter G, Hart Adam H, Bagheri-Fam Stefan, Wallace Megan J, Dobbie Michael S, Gordon Christopher T, Miller Kerry A, Whittle Belinda, Abud Helen E, Arkell Ruth M, Cole Timothy J, Harley Vincent R, Smyth Ian M, Bertram John F
Abstract excerpt
BACKGROUND: Mice harbouring gene mutations that cause phenotypic abnormalities during organogenesis are invaluable tools for linking gene function to normal development and human disorders. To generate mouse models harbouring novel alleles that are involved in organogenesis we conducted a phenotype-driven, genome-wide mutagenesis screen in mice using the mutagen N-ethyl-N-nitrosourea (ENU). METHODOLOGY/PRINCIPAL...
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