Article
Increased functional stability and homogeneity of viral envelope spikes through directed evolution.
PLoS pathogens - 1 Feb 2013
Leaman Daniel P, Zwick Michael B
Abstract excerpt
The functional HIV-1 envelope glycoprotein (Env) trimer, the target of anti-HIV-1 neutralizing antibodies (Abs), is innately labile and coexists with non-native forms of Env. This lability and heterogeneity in Env has been associated with its tendency to elicit non-neutralizing Abs. Here, we use directed evolution to overcome instability and heterogeneity of a primary Env spike. HIV-1 virions were subjected to...
Topics
- Antibodies, Viral
- Cell Line
- Cloning, Molecular
- Directed Molecular Evolution
- HIV Envelope Protein gp120
- HIV-1
- Humans
- Mutation
- Protein Denaturation
- Urea
- Virion
- env Gene Products, Human Immunodeficiency Virus