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Article

Increased functional stability and homogeneity of viral envelope spikes through directed evolution.

PLoS pathogens - 1 Feb 2013

Leaman Daniel P, Zwick Michael B

Abstract excerpt

The functional HIV-1 envelope glycoprotein (Env) trimer, the target of anti-HIV-1 neutralizing antibodies (Abs), is innately labile and coexists with non-native forms of Env. This lability and heterogeneity in Env has been associated with its tendency to elicit non-neutralizing Abs. Here, we use directed evolution to overcome instability and heterogeneity of a primary Env spike. HIV-1 virions were subjected to...

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Share to Antibodies, Viral
Share to Directed Molecular Evolution
Share to env Gene Products, Human Immunodeficiency Virus

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to HIV Envelope Protein gp120

Share to Protein Denaturation

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Source

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Identifiers

PMID: 23468626
DOI: 10.1371/journal.ppat.1003184
PMCID: PMC3585149

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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