Article
DNA analysis using polymerase chain reactions in the families of children with cystic fibrosis.
The New Zealand medical journal - 9 May 1990
Sullivan M J, Myles T, Murphy J, George P, Dawson K P
Abstract excerpt
The results of our experience in DNA analysis in cystic fibrosis families using polymerase chain reaction (PCR) is reported. Of the first 10 families studied, seven were fully informative using the probe KM19 and PCR. In the remaining three families, Southern blotting analysis and the probe pXV2c were required before full information was obtained in two and partial information in the other family. The methods...
Topics
- Blotting, Southern
- Child
- Cystic Fibrosis
- DNA
- DNA Probes
- Female
- Genotype
- Humans
- Male
- Pedigree
- Polymerase Chain Reaction