Article
Cystic fibrosis marker testing in Bohemia with polymerase chain reaction.
Acta Universitatis Carolinae. Medica - 1 Jan 1990
Macek M, Böhm I, Arnold L, Smrt J, Duspivová R, Vávrová V, Macek M, Jelínková E, Schmidtke J
Abstract excerpt
Conditions for assessing KM-19 probe detected by Pst-1 restriction fragment length polymorphism (RFLP) by means of polymerase chain reaction were provided. Computer controlled mechanical arm with waterbaths and cloned heat-stable DNA polymerase was used. Results of KM-19 allelic frequencies on 90 cystic fibrosis chromosomes are presented. Allele two frequency was -0.833.
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.