Article
Cystic fibrosis marker testing in Bohemia with polymerase chain reaction.
Acta Universitatis Carolinae. Medica - 1 Jan 1990
Macek M, Böhm I, Arnold L, Smrt J, Duspivová R, Vávrová V, Macek M, Jelínková E, Schmidtke J
Abstract excerpt
Conditions for assessing KM-19 probe detected by Pst-1 restriction fragment length polymorphism (RFLP) by means of polymerase chain reaction were provided. Computer controlled mechanical arm with waterbaths and cloned heat-stable DNA polymerase was used. Results of KM-19 allelic frequencies on 90...
Topics
- Alleles
- Cystic Fibrosis
- DNA
- Female
- Gene Frequency
- Genetic Markers
- Humans
- Polymerase Chain Reaction
- Pregnancy
- Prenatal Diagnosis
