Detection of delta F508 cystic fibrosis mutation by polymerase chain reaction from old paraffin-embedded tissues: a retrospective autopsy study.

Article

Detection of delta F508 cystic fibrosis mutation by polymerase chain reaction from old paraffin-embedded tissues: a retrospective autopsy study.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc - 1 Apr 1994

Palacios J, Ezquieta B, Gamallo C, Limeres M A, Benito N, Rodriguez J I, Molano J

Abstract excerpt

This study analyzes the usefulness of the polymerase chain reaction technique in the detection of delta F508 mutation in 11- to 25-yr-old formalin-fixed paraffin-embedded tissues obtained from the autopsy of 38 cystic fibrosis patients (nine with meconium ileus). Two different pairs of oligonucle...

Topics

Base Sequence
Cystic Fibrosis
DNA Mutational Analysis
DNA Primers
Electrophoresis, Agar Gel
Formaldehyde
Humans
Infant, Newborn
Meconium
Molecular Sequence Data
Mutation

Paraffin Embedding

Polymerase Chain Reaction

Retrospective Studies

Tissue Fixation

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