Article
Comparative induction of gene mutations and chromosome damage by 1-methoxy-1,3,5-cycloheptatriene (MCHT), 2. Results using L5178Y mouse lymphoma cells to detect both gene and chromosome damage; validation with ionizing radiation, methyl methanesulphonate, ethyl methanesulphonate and benzo[a]pyrene.
Mutation research - 1 May 1990
Cole J, Diot M C, Richmond F N, Bridges B A
Abstract excerpt
A variation of the mouse lymphoma (L5178Y TK+/(-)-3.7.2c) assay has been developed using a microtiter cloning technique instead of the standard agar method. The cell line has been used to detect both gene mutations (at the Na+/K+ ATPase and thymidine kinase loci) and chromosome damage (micronucleus induction) in the same experiment. The system was validated using gamma-irradiation (a known clastogen), 2...
Topics
- Animals
- Benzo(a)pyrene
- Chromosome Aberrations
- Chromosomes
- Cloning, Molecular
- Cycloheptanes
- DNA Damage
- Ethyl Methanesulfonate
- Genes
- Lymphoma