Article
Multistage neoplastic transformation of Syrian hamster embryo cells cultured at pH 6.70.
Cancer research - 15 Jun 1990
LeBoeuf R A, Kerckaert G A, Aardema M J, Gibson D P
Abstract excerpt
We have reported that the frequency of induction of the morphologically transformed phenotype in early passage Syrian hamster embryo cells is increased in carcinogen-treated cultures when the cells are cultured in media of pH 6.70, instead of the traditionally used pH 7.35. The purpose of the studies reported here was to determine the neoplastic potential of cells derived from colonies of morphologically normal,...
Topics
- Animals
- Benzo(a)pyrene
- Cell Count
- Cell Line
- Cell Transformation, Neoplastic
- Cricetinae
- Embryo, Mammalian
- Hydrogen-Ion Concentration
- Karyotyping
- Mesocricetus
- Methylcholanthrene