Article
Comparison of COBAS 4800 KRAS, TaqMan PCR and high resolution melting PCR assays for the detection of KRAS somatic mutations in formalin-fixed paraffin embedded colorectal carcinomas.
Virchows Archiv : an international journal of pathology - 1 Mar 2013
Harlé Alexandre, Busser Benoit, Rouyer Marie, Harter Valentin, Genin Pascal, Leroux Agnès, Merlin Jean-Louis
Abstract excerpt
Many studies documented the influence of KRAS mutation status on the response of patients with metastatic colorectal cancer (mCRC) to anti-EGFR monoclonal antibodies. The COBAS 4800 KRAS is an assay using real time PCR and TaqMelt technology, CE-IVD validated, for the detection of 19 KRAS somatic mutations in exons 2 and 3. We compared COBAS with previously validated PCR TaqMan and High Resolution Melting (HRM)...
Topics
- Adenocarcinoma
- Adult
- Aged
- Aged, 80 and over
- Colorectal Neoplasms
- Female
- Formaldehyde
- Humans
- Male
- Middle Aged
- Mutation
- Paraffin Embedding
- Polymerase Chain Reaction