Article
Investigation of activity of recombinant mengovirus RNA-dependent RNA polymerase and its mutants.
Biochemistry. Biokhimiia - 1 Jan 2013
Shatskaya G S, Drutsa V L, Koroleva O N, Osterman I A, Dmitrieva T M
Abstract excerpt
The activities of wild-type mengovirus RNA polymerase (RdRP) and of its three mutants with C-terminal tryptophan residue replaced by residues of alanine (W460A), phenylalanine (W460F), or tyrosine (W460Y) were studied. The proteins were expressed in E. coli and purified by affinity chromatography with the IMPACT system. The isolated recombinant proteins were studied using a cell-free replication system on...
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