Article
Temperature requirements for initiation of RNA-dependent RNA polymerization.
Virology - 30 Sept 2003
Yang Hongyan, Gottlieb Paul, Wei Hui, Bamford Dennis H, Makeyev Eugene V
Abstract excerpt
To continue the molecular characterization of RNA-dependent RNA polymerases of dsRNA bacteriophages (Cystoviridae), we purified and biochemically characterized the wild-type (wt) and a temperature-sensitive (ts) point mutant of the polymerase subunit (Pol) from bacteriophage phi12. Interestingly, initiation by both wt and the ts phi12 Pol was notably more sensitive to increased temperatures than the elongation...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.