Article
Protein-DNA interactions upstream from the human A gamma globin gene.
Nucleic acids research - 25 Apr 1990
O'Neill D, Kaysen J, Donovan-Peluso M, Castle M, Bank A
Abstract excerpt
We have used DNAase I footprinting and the gel mobility shift assay to study proteins which bind to promoter elements located between -140 and -382 upstream of the human A gamma globin gene. Footprints are found with both erythroid and nonerythroid nuclear extracts at three sites: from -294 to -264, -242 to -227, and -189 to -172 from the transcription initiation site. An erythroid-specific footprint is...
Topics
- Base Sequence
- Binding Sites
- Binding, Competitive
- Cell Line
- DNA
- DNA-Binding Proteins
- Deoxyribonuclease I
- Erythroid-Specific DNA-Binding Factors
- GATA1 Transcription Factor
- Globins
- Host Cell Factor C1